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Publication Details
Output Category: D
Strategic Research Area: None
TISI Citations: PlumX StatisticsPlumX Statistics
Scopus Citations:
Journal Impact: 15.68
All Authors: Mackay, J, Landsberg, M, Whitten, A, Bond, C Number:
UWA Authors: Bond, C. S. Number: 1
Title: Whaddaya Know: A Guide to Uncertainty and Subjectivity in Structural Biology
ISBN/ISSN 0968-0004
Year: 2017
Pages: 155-167
Volume: 42
Issue: 2
Full Reference (Harvard Style): Mackay, J. P., Landsberg, M. J., Whitten, A. E., Bond, C. S. 2017, 'Whaddaya Know: A Guide to Uncertainty and Subjectivity in Structural Biology', Trends in Biochemical Sciences, 42, 2, pp. 155-167.

The methods of structural biology, while powerful, are technically complex. Although the Protein Data Bank (PDB) provides a repository that allows anyone to download any structure, many users would not appreciate the caveats that should be considered when examining a structure. Here, we describe several key uncertainties associated with the application of X-ray crystallography, NMR spectroscopy, single-particle electron microscopy (SPEM), and small-angle scattering (SAS) to biological macromolecules. The take-home message is that structures are not absolute truths – they are models that fit the experimental data and therefore have uncertainty and subjectivity associated with them. These uncertainties must be appreciated – careful reading of the associated paper, and any validation report provided by the structure database, is highly recommended.